Preservation of strain registration
- Registration request
- Receipt/Strain Confirmation
- Determining compliance with registration criteria
- Culture
- Quality test
- Report/Approval (team leader)
- Barcode registration
- Computer registration of strain information
- Long-term preservation of strains
- Regeneration test
- Release of microbial information
- Sending the microorganism registration number notice
- Reporting registration result (director)
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- 1. Biochemical characteristics
- 2. Effective component productivity and quantification
- 3. Identification (checking strains)-PCR, Realtime PCR
- Registration category
- - All bacteria except for human and animal pathogens belonging to the
European hazard rating 3 and 4 groups - - All yeasts and molds, regardless of hazard rating
- - All bacteria except for human and animal pathogens belonging to the
- Characteristics of registered microorganisms
- - Microorganisms that are different from MIFI-holding strains
- - Academically important microorganisms such as standard strains
and reference strains - - Microorganisms with fermentation ability
- - Economically important microorganisms due to material
production, etc. - - Microorganisms that are important to R&D of the Microbial
Institute for Fermentation Industry
- Basic conditions of registered microorganisms
- - Microorganisms with adequate information
- - Pure strains or microorganisms which have been identified
- - Microorganisms that can be cultured in artificial media
- - Microorganisms that can be preserved by lyophilization or
cryopreservation in liquid nitrogen without significant changes in characteristics.
Strain preservation management
Microorganism preservation technique
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Lyophilization
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Freezer preservation
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Liquid nitrogen preservation
(straw preservation) -
Mineral oil preservation
Application of preservation method for each microorganism
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Bacteria, Spore-forming mold
- - Drying (lyophilization, liquid drying)
- - Freezing (liquid nitrogen preservation, low-temperature freezer preservation)
-
Spore-non-forming mold
- - Active state preservation (sharing preservation, water preservation)
- - Freezing (liquid nitrogen preservation, low-temperature freezer preservation)
The registered microorganisms are preserved in two or more ways.At least one of the methods is a liquid nitrogen preservation or lyophilization with little genetic variation.
